Poster 6: Thi-Ut Nguyen
National Pediatric Hospital, Vietnam
Title: Evidence of intra- familial of Helicobacter pylori by PCR- based RAPD fingerprinting in Vietnam
Biography
Biography: Poster 6: Thi-Ut Nguyen
Abstract
Statement of the problem: Helicobacter pylori (H. pylori) is the major cause of gastritis and peptic ulcer disease in children. Transmission between familiy members could be increasing risks of reinfection in children. The objective of this study is to assessed transmission by DNA fingerprinting analysis of H. pylori culture from pediatric patients and their family members. Methodology & Theoretical Orientation: We selected 17 households with H. pylori patients. 50 H. pylori strains were isolated from patients and family members, of whom 17 were from pediatric patients, 33 from family members including fathers, mothers and brothers/sisters. All 50 H. pylori strains were analyzed by RAPD technique between January, 2012 through September, 2013 in National hospital of pediatrics, Hanoi, Vietnam. Findings: Results show that 46.1% (6/13) mothers had genotypes similar to the ones of their children. In contrast, only 8.3% (1/12) fathers had genotypes similar to the ones of their children. 11.8% (2/17) H. pylori strains of pediatric patients were similar to the ones of their brothers/sisters. However, only one strain of fathers and mothers have similar genes but is not identical to their offspring. Conclusion & Significance: The results show that there is a link between the mother-to-child isolate, which may be evidence of mother-to-child transmission, especially in young children. The results of our study provide further proof of the molecular level of H. pylori transmission between family members in Viet Nam. further studies on H. pylori transmission among family members with larger sample sizes are needed.